Beta 2M-/- knockout mice contain low levels of CD8+ cytotoxic T lymphocyte that mediate specific tumor rejection.

摘要: C57BL/6 mice with a disrupted beta 2M gene (beta 2M-/- mice) express very low levels of MHC class I molecules and are deficient for CD8+ T lymphocytes. Because cells thought to be principle effector cell in tumor rejection, we have assessed the ability respond tumors. knockout were challenged seven independent allogeneic syngeneic The responded similarly their 2M+/- littermates that they rejected high dose challenges 4/5 tumors susceptible 3/3 In vivo depletion CD4+ or from resulted susceptibility tumor. apparent requirement immunity was corroborated by vitro assays which but not eliminated tumor-specific CTL activity. mAb blocking studies target incubated I-specific demonstrated activity restricted. therefore contain small quantities potent, capable rejecting large cells.