Knockdown of long non-coding RNA MEG3 protects H9c2 cells from hypoxia-induced injury by targeting microRNA-183.
作者： Licheng Gong , Haiming Xu , Hong Chang , Yaliang Tong , Tao Zhang
DOI: 10.1002/JCB.26304
关键词:
摘要: Acute myocardial infarction (AMI) occurs when blood supply to the heart is diminished (ischemia) for long time, and ischemia primarily caused due hypoxia. This study evaluated effects of non-coding RNA maternally expressed gene 3 (MEG3) on hypoxic rat cardiomyocyte-drived H9c2 cells. Hypoxic injury was confirmed by alterations cell viability, migration, invasion, apoptosis, hypoxia-inducible factor 1α (HIF-1α) expression. MEG3 level in cells its knockdown were assessed. The interactions between miR-183 as well p27 investigated. In addition, aberrantly MEG3, miR-183, along with activation PI3K/AKT/FOXO3a signaling pathway all Results showed that hypoxia induced decreases migration increases apoptosis expressions HIF-1α MEG3. Knockdown decreased hypoxia-induced also increased expression, which identified a target reversed silence. expression negatively regulated miR-183. mechanistic studies revealed activating signal pathways. These findings suggest alleviates miR-183-mediated suppression through pathway.
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