In vitro screening for compounds from Hypericum longistylum with anti-pulmonary fibrosis activity.

摘要: Abstract Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with poor prognosis and limited therapies, transforming growth factor-β1 (TGF-β1) plays central role in the pathogenesis of IPF. Here, we aimed to investigate chemical constituents biological activities Hypericum longistylum detect whether isolated compounds inhibit TGF-β1/Smad3 signaling pathway identify candidate for treatment fibrosis. Fifteen (1–15) were from H. their structures elucidated on basis spectroscopic analyses. An vitro MTT assay was used test effect these fifteen fibroblast cytotoxicity vitality. Furthermore, bioactivities screened using luciferase reporter vitro. screening found that 1–15 had no deleterious effects normal mouse fibroblasts significant inhibition Luciferase showed 14 15 could significantly rates 67.92% 93.10%, respectively. Both can be as lead structural modification optimization obtain more drug candidates