Overexpression of glucosylceramide synthase in associated with multidrug resistance of leukemia cells
作者： Ping Xie , Yun-Fen Shen , Yuan-Ping Shi , Shu-Mei Ge , Zhong-Hua Gu
DOI: 10.1016/J.LEUKRES.2007.07.006
关键词:
摘要: Abstract Ceramide, as a second messenger, initiates one of the major signal transduction pathways in tumor apoptosis. Glucosylceramide synthase (GCS) catalyzes glycosylation ceramide and produces glucosylceramide. Through GCS, allows cellular escape from ceramide-induced programmed cell death. Here we investigated expression GCS human leukemia cells an association between multidrug resistance cells. Using RT-PCR technique level gene was detected 65 clinical resistance/non-resistance cases with leukemia, K562 K562/A02 lines. AlamarBlue Assay applied to confirm resistant PPMP, which is chemical inhibitor for used determine relationship drug-resistance In addition, (mdr1), Bcl-2 Bax mRNA also analyzed by RT-PCR. The mdr1 clinic samples exhibited significantly increased compared drug sensitive group (P
elsevier.com
sci-hub.se 参考文章(26)
Emmaneul CORONEOS, Yizheng WANG, James R. PANUSKA, Dennis J. TEMPLETON, Mark KESTER, Sphingolipid metabolites differentially regulate extracellular signal-regulated kinase and stress-activated protein kinase cascades. Biochemical Journal. ,vol. 316, pp. 13- 17 ,(1996) , 10.1042/BJ3160013
R.T. Dobrowsky, C. Kamibayashi, M.C. Mumby, Y.A. Hannun, Ceramide activates heterotrimeric protein phosphatase 2A. Journal of Biological Chemistry. ,vol. 268, pp. 15523- 15530 ,(1993) , 10.1016/S0021-9258(18)82288-8
Kurt C. Almquist, David R. Hipfner, Roger G. Deeley, Susan P. C. Cole, Caroline E. Grant, Gunnar Valdimarsson, Overexpression of multidrug resistance-associated protein (MRP) increases resistance to natural product drugs. Cancer Research. ,vol. 54, pp. 357- 361 ,(1994)
J. Liu, S. Mathias, Z. Yang, R.N. Kolesnick, Renaturation and tumor necrosis factor-alpha stimulation of a 97-kDa ceramide-activated protein kinase. Journal of Biological Chemistry. ,vol. 269, pp. 3047- 3052 ,(1994) , 10.1016/S0021-9258(17)42045-X
Nandigama Krishnamachary, The MRP Gene Associated with a Non-P-glycoprotein Multidrug Resistance Encodes a 190-kDa Membrane Bound Glycoprotein Cancer Research. ,vol. 53, pp. 3658- 3661 ,(1993)
Anton G. Borg, Robert Burgess, Linda M. Green, Rik J. Scheper, John A. Liu Yin, Overexpression of lung‐resistance protein and increased P‐glycoprotein function in acute myeloid leukaemia cells predict a poor response to chemotherapy and reduced patient survival British Journal of Haematology. ,vol. 103, pp. 1083- 1091 ,(1998) , 10.1046/J.1365-2141.1998.01111.X
Manfred Volm, Michael Kästel, JÜRgen Mattern, Thomas Efferth, Expression of resistance factors (P-glycoprotein, glutathione S-transferase-π, and topoisomerase II) and their interrelationship to proto-oncogene products in renal cell carcinomas Cancer. ,vol. 71, pp. 3981- 3987 ,(1993) , 10.1002/1097-0142(19930615)71:12<3981::AID-CNCR2820711231>3.0.CO;2-A
Branimir I Sikic, George A Fisher, Bert L Lum, Joanne Halsey, Lidija Beketic-Oreskovic, Gang Chen, Modulation and prevention of multidrug resistance by inhibitors of P-glycoprotein. Cancer Chemotherapy and Pharmacology. ,vol. 40, ,(1997) , 10.1007/S002800051055
A. Di Nardo, L. Benassi, C. Magnoni, A. Cossarizza, S. Seidenari, A. Giannetti, Ceramide 2 (N-acetyl sphingosine) is associated with reduction in Bcl-2 protein levels by Western blotting and with apoptosis in cultured human keratinocytes. British Journal of Dermatology. ,vol. 143, pp. 491- 497 ,(2000) , 10.1111/J.1365-2133.2000.03700.X
Y Liu, Glycosylation of ceramide potentiates cellular resistance to tumor necrosis factor-alpha-induced apoptosis. Experimental Cell Research. ,vol. 252, pp. 464- 470 ,(1999) , 10.1006/EXCR.1999.4649