Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated.

摘要: Iron is essential for the proliferation of fungal pathogens during infection. The availability iron limited due to its association with host proteins. Fungal have evolved different mechanisms acquire from host; however, little known regarding how Paracoccidioides species incorporate and metabolize this ion. In work, sources that are used by spp. were investigated. Robust growth in presence iron-containing molecules hemin hemoglobin was observed. present hemolytic activity ability internalize a protoporphyrin ring. Using real-time PCR nanoUPLC-MSE proteomic approaches, shown result positive regulation transcripts encode putative receptors, addition induction proteins required amino acid metabolism vacuolar protein degradation. fact, one receptor ortholog, Rbt5, identified as surface GPI-anchored recognized hemin, vitro. Antisense RNA technology Agrobacterium tumefaciens-mediated transformation generate mitotically stable Pbrbt5 mutants. knockdown strain had lower survival inside macrophages mouse spleen when compared parental strain, which suggested Rbt5 could act virulence factor. summary, our data indicate can use an source most likely through receptor-mediated pathways might be relevant pathogenic mechanisms.