The reconstituted carnitine carrier from rat liver mitochondria: evidence for a transport mechanism different from that of the other mitochondrial translocators
作者： Cesare Indiveri , Annamaria Tonazzi , Ferdinando Palmieri
DOI: 10.1016/0005-2736(94)90281-X
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摘要: The transport mechanism of the reconstituted carnitine carrier purified from rat liver mitochondria was investigated kinetically. half-saturation constant (Km) for on internal side liposomal membrane (8.7 mM) found to be much higher than that determined external surface (0.45 mM). exclusive presence a single affinity each indicated unidirectional insertion into proteoliposomes, most probably right-side-out with respect mitochondria. Under these defined conditions bisubstrate initial velocity studies homologous (carnitine/carnitine) and heterologous (carnitine/acylcarnitine) antiport were performed by varying both substrate concentrations. kinetic patterns obtained showed ratio Km/Vmax is not influenced second (non-varied) substrate, which indicates ping-pong mechanism. thus differs all other mitochondrial carriers analyzed so far in state, common sequential type reaction has been found.
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sci-hub.se 参考文章(29)
H. Aquila, T.A. Link, M. Klingenberg, The uncoupling protein from brown fat mitochondria is related to the mitochondrial ADP/ATP carrier. Analysis of sequence homologies and of folding of the protein in the membrane. The EMBO Journal. ,vol. 4, pp. 2369- 2376 ,(1985) , 10.1002/J.1460-2075.1985.TB03941.X
M. J. Runswick, S. J. Powell, P. Nyren, J. E. Walker, Sequence of the bovine mitochondrial phosphate carrier protein: structural relationship to ADP/ATP translocase and the brown fat mitochondria uncoupling protein. The EMBO Journal. ,vol. 6, pp. 1367- 1373 ,(1987) , 10.1002/J.1460-2075.1987.TB02377.X
Ferdinando Palmieri, Martin Klingenberg, [26] Direct methods for measuring metabolite transport and distribution in mitochondria Methods in Enzymology. ,vol. 56, pp. 279- 301 ,(1979) , 10.1016/0076-6879(79)56029-7
M S Murthy, S V Pande, Mechanism of carnitine acylcarnitine translocase-catalyzed import of acylcarnitines into mitochondria. Journal of Biological Chemistry. ,vol. 259, pp. 9082- 9089 ,(1984) , 10.1016/S0021-9258(17)47268-1
C Indiveri, R Krämer, F Palmieri, Reconstitution of the malate/aspartate shuttle from mitochondria. Journal of Biological Chemistry. ,vol. 262, pp. 15979- 15983 ,(1987) , 10.1016/S0021-9258(18)47685-5
R Parvin, S V Pande, Enhancement of mitochondrial carnitine and carnitine acylcarnitine translocase-mediated transport of fatty acids into liver mitochondria under ketogenic conditions. Journal of Biological Chemistry. ,vol. 254, pp. 5423- 5429 ,(1979) , 10.1016/S0021-9258(18)50613-X
S.V. Pande, R. Parvin, Carnitine-acylcarnitine translocase catalyzes an equilibrating unidirectional transport as well Journal of Biological Chemistry. ,vol. 255, pp. 2994- 3001 ,(1980) , 10.1016/S0021-9258(19)85841-6
R Parvin, S V Pande, Pyruvate and acetoacetate transport in mitochondria. A reappraisal. Journal of Biological Chemistry. ,vol. 253, pp. 1565- 1573 ,(1978) , 10.1016/S0021-9258(17)34903-7
O. Fröhlich, R.B. Gunn, Erythrocyte anion transport: the kinetics of a single-site obligatory exchange system Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes. ,vol. 864, pp. 169- 194 ,(1986) , 10.1016/0304-4157(86)90010-9
Thomas Dierks, Etta Riemer, Reinhard Krämer, Reaction mechanism of the reconstituted aspartate/glutamate carrier from bovine heart mitochondria. Biochimica et Biophysica Acta. ,vol. 943, pp. 231- 244 ,(1988) , 10.1016/0005-2736(88)90555-X