2'-Azido RNA, a versatile tool for chemical biology: synthesis, X-ray structure, siRNA applications, click labeling.

摘要: Chemical modification can significantly enrich the structural and functional repertoire of ribonucleic acids endow them with new outstanding properties. Here, we report syntheses novel 2′-azido cytidine guanosine building blocks demonstrate their efficient site-specific incorporation into RNA by mastering synthetic challenge using phosphoramidite chemistry in presence azido groups. Our study includes detailed characterization nucleoside containing UV-melting profile analysis CD NMR spectroscopy. Importantly, X-ray crystallographic uridine adenosine modified RNAs reveals crucial details this within an A-form double helical environment. The group supports C3′-endo ribose conformation shows distinct water-bridged hydrogen bonding patterns minor groove. Additionally, siRNA induced silencing brain acid soluble protein (BASP1) encoding gene i...