Calcium-dependent activation of mitochondrial metabolism in mammalian cells.

摘要: Endogenous fluorophores provide a simple, but elegant means to investigate the relationship between agonist-evoked Ca2+ signals and activation of mitochondrial metabolism. In this article, we discuss methods strategies measure cellular pyridine nucleotide flavoprotein fluorescence alone or in combination with Ca2+-sensitive indicators. These were developed using primary cultured hepatocytes neurons, which contain relatively high levels endogenous robust metabolic responses. Nevertheless, these are amendable wide variety cell types lines that maintain active