Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata.

摘要: Although microalgal biofuels have potential advantages over conventional fossil fuels, high production costs limit their application in the market. We developed bio-flocculation and incubation methods for marine alga, Nannochloropsis oceanica CCMP1779, oleaginous fungus, Mortierella elongata AG77, resulting increased oil productivity. By growing separately then combining cells, M. mycelium could efficiently capture N. due to an intricate cellular interaction between two species leading bio-flocculation. Use of a high-salt culture medium induced accumulation triacylglycerol (TAG) enhanced contents polyunsaturated fatty acids (PUFAs) including arachidonic acid docosahexaenoic elongata. To increase TAG productivity we effective, reduced nitrogen-supply regime based on ammonium environmental photobioreactors. Under optimized conditions, produced levels that be indirectly monitored by following chlorophyll content. Combining initiate yielded total acids, with ~ 15 22% dry weight (DW), respectively, as well PUFAs. Genetic engineering higher content nutrient-replete was accomplished overexpressing DGTT5, gene encoding type II acyl-CoA:diacylglycerol acyltransferase 5. Combined bio-flocculation, this approach led under conditions (~ 10% DW) compared wild (~ 6% DW). The combined use available genomes genetic tools both opens up new avenues improve biofuel allows acids.