Method for the purification of erythropoietin and erythropoietin compositions
作者： Rodney M. Hewick , Jasbir S. Seehra
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摘要: A method for purifying erythropoietin is described. The comprises treating partially by reverse phase high performance liquid chromatography to obtain homogeneous having a molecular weight of about 34,000 daltons on SDS PAGE and moving single peak HPLC. protein preferably has specific activity at least 120,000 IU, more 160,000 IU per absorbance unit 280 nm.
lens.org 参考文章(15)
White Wilfrid F, Schlueter Robert J, Erythropoietic factor purification ,(1959)
Ryuzo Sasaki, Hideo Chiba, Masatsugu Ueda, Process for producing erythropoietin ,(1983)
Hajime Hiratani, Kenji Takezawa, Process for production of erythropoietin ,(1980)
Sylvia Lee-Huang, Reverse immunoaffinity chromatography purification method ,(1984)
Kenji Takezawa, Hajima Hiratani, Novel erythropoietin product and method for the preparation thereof ,(1982)
Clifford Kavinsky, Tania L. Weiss, Eugene Goldwasser, Monoclonal anti-erythropoietin ,(1982)
Jean E. F. Rivier, Mobile phase for liquid chromatography ,(1978)
Joan C. Egrie, A.T.C.C. HB8209 and its monoclonal antibody to erythropoietin ,(1983)
Sidney Pestka, Menachem Rubinstein, Protein purification process and product ,(1983)
Kaname Sugimoto, Yasushi Hayashibara, Process for the production of human erythropoietin ,(1981)