Gas chromatographic-mass spectrometric assay of four indole alkylamines of rat pineal.
作者： F. Cattabeni , S. H. Koslow , E. Costa
DOI: 10.1126/SCIENCE.178.4057.166
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摘要: Gas chromatography-mass spectrometry was used to quantitate serotonin, N- acetylserotonin, 5-methoxytryptamine, and melatonin in single rat pineal glands. After gas chromatographic separation, the ion density of specific fragments each indole measured with mass spectrometry. Sensitivity this assay is order 10 -12 -13 mole. Routinely, specificity based on retention time recording generated by fragments. Absolute identification extracted indoles multiple detection.
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