Method of identifying cytosine methylation patterns in genomic DNA samples
作者： Alexander Olek
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摘要: The invention relates to a method of identifying cytosine methylation patterns in genomic DNA samples. inventive comprises the following steps: a) chemically treating sample such manner that and 5-methyl react differently two products have different base-pairing behavior duplex; b) enzymatically amplifying parts treated this manner; c) binding amplified surface; d) hybridizing set probes nucleic base sequences immobilized samples, said respective containing at least once dinucleotide sequence 5'-CpG-3'; e) removing non-hybridized probes; f) analyzing hybridized mass spectrometer, position on carrier allowing an allocation be hybridized; g) converting peak-patterns obtained from mass-specters matching new data with library.
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