Stochastic simulations of a synthetic bacteria-yeast ecosystem

摘要: The field of synthetic biology has greatly evolved and numerous functions can now be implemented by artificially engineered cells carrying the appropriate genetic information. However, in order for to robustly perform complex or multiple tasks, co-operation between them may necessary. Therefore, various biological systems whose functionality requires cell-cell communication are being designed. These systems, microbial consortia, composed exhibit a wide range behaviors. include yeast growth is dependent on one another, bacteria that kill rescue each other, synchronize, behave as predator-prey ecosystems invade cancer cells. In this paper, we study ecosystem comprising communicate with benefit from other using small diffusible molecules. We explore behavior heterogeneous consortium, Saccharomyces cerevisiae Escherichia coli cells, stochastic modeling. model captures relevant intra-cellular inter-cellular interactions taking place eukaryotic prokaryotic Integration well-characterized molecular regulatory elements into these two microbes allows through quorum sensing. A gene controlling induced via chemical signals vice versa. Interesting dynamics common natural ecosystems, such obligatory facultative mutualism, extinction, commensalism like observed. investigate report conditions under which species successfully other. This explores behaviors exhibited cohabitation bacterial way built studying any system consisting communicating another signals. key information acquired our potentially drive experimental design ecosystems.