Understanding endothelial glycocalyx function under flow shear stress from a molecular perspective.

摘要: BACKGROUND: The endothelial glycocalyx plays a pivotal role in regulating blood flow, filtering components, sensing and transducing mechanical signals. These functions are intimately related to its dynamics at the molecular level. OBJECTIVE: objective of this research is establish relationship between METHODS: To such relationship, large-scale simulations were undertaken mimic components presence flow shear stresses. RESULTS: First, motions core protein pertinent subdomains scrutinised. Three-directional movements observed, although was imposed only x direction. Such an observation contributes understanding redistribution as reported experiments. Unsynchronised motion also spotted, which provides alternative explanation macroscopic phenomena. Moreover, dynamics, root-mean-square-deviations conformational changes sugar chains investigated. Based on findings, force transmission pathway, chains, potential influence signalling transduction pathway proposed discussed. CONCLUSIONS: This study relates with microscopic fills knowledge gap about links different scales.