Achillea millefolium L. hydroethanolic extract inhibits growth of human tumor cell lines by interfering with cell cycle and inducing apoptosis.
作者： Joana M. Pereira , Vanessa Peixoto , Alexandra Teixeira , Diana Sousa , Lillian Barros
DOI: 10.1016/J.FCT.2018.06.006
关键词:
摘要: Abstract The cell growth inhibitory activity of the hydroethanolic extract Achillea millefolium was studied in human tumor lines (NCI-H460 and HCT-15) its mechanism action investigated. GI50 concentration determined with sulforhodamine B assay cycle apoptosis were analyzed by flow cytometry following incubation PI or Annexin V FITC/PI, respectively. expression levels proteins involved Western blot. extracts characterized regarding their phenolic composition LC-DAD-ESI/MS. 3,5-O-Dicaffeoylquinic acid, followed 5-O-caffeoylquinic main acids, while, luteolin-O-acetylhexoside apigenin-O-acetylhexoside flavonoids. This decreased tested lines, being more potent HCT-15 then NCI-H460 cells. Two different concentrations (75 100 μg/mL) caused alterations profile increased Moreover, an increase p53 p21 NCI-H460 cells (which have wt p53), reduced XIAP HCT-15 cells (with mutant p53). work enhances importance A. as source bioactive compounds, particularly inhibitors.
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