Comparison of Biological Effects on Human Keratinocytes Using Different Plasma Treatment Regimes

摘要: This study investigated the influence of plasma treatment regime on human keratinocytes (HaCaT cells). HaCaT cells were treated with a volume dielectric barrier discharge source in three different ways: directly, directly culture medium exchange, and indirectly (in which only cell was exposed to plasma). The viability, DNA, cycle, intracellular concentration reactive oxygen species investigated. Direct indirect caused time–dependent decrease viable cells. An increase DNA damage observed immediately after treatment, diminished 24 h. Intracellular increased longer times. cycle analysis showed an accumulation G2/M phase at expense G1 phase. immediate exchange attenuated described effects. adherent resulted comparable effects that depend time. Physical seems generate long-living or modify organic components medium. Both mechanisms can initiate oxidative stress keratinocytes, is responsible for