Role for DNA Polymerase κ in the Processing of N2-N2-Guanine Interstrand Cross-links
作者： Irina G. Minko , Michael B. Harbut , Ivan D. Kozekov , Albena Kozekova , Petra M. Jakobs
关键词:
摘要: Although there exists compelling genetic evidence for a homologous recombination-independent pathway repair of interstrand cross-links (ICLs) involving translesion synthesis (TLS), biochemical support this model is lacking. To identify DNA polymerases that may function in TLS past ICLs, oligodeoxynucleotides were synthesized containing site-specific ICLs which the linkage was between N2-guanines, similar to formed by mitomycin C and enals. Here, data are presented mammalian cell replication DNAs these lesions ∼97% accurate. Using series mimic potential intermediates ICL repair, we demonstrate human polymerase (pol) κ not only catalyzed accurate incorporation opposite cross-linked guanine but also replicated beyond lesion, thus providing first an ICL. The efficiency greatly enhanced truncation both 5 ′ 3 ends nontemplating strand. Further analyses showed although yeast Rev1 could incorporate dCTP guanine, no found pol ζ or ζ/Rev1 combination. Because able bypass biological role tolerating N2-N2-guanine sought; survival chromosomal stability adversely affected κ-depleted cells following exposure. Thus, cellular studies suggest processing ICLs.
elsevier.com
sci-hub.se 参考文章(46)
Tomoo Ogi, Alan R. Lehmann, The Y-family DNA polymerase κ (pol κ) functions in mammalian nucleotide-excision repair Nature Cell Biology. ,vol. 8, pp. 640- 642 ,(2006) , 10.1038/NCB1417
Steven Creighton, Linda B. Bloom, Myron F. Goodman, Gel fidelity assay measuring nucleotide misinsertion, exonucleolytic proofreading, and lesion bypass efficiencies. Methods in Enzymology. ,vol. 262, pp. 232- 256 ,(1995) , 10.1016/0076-6879(95)62021-4
E. Moustacchi, J. A. P. Henriques, ISOLATION AND CHARACTERIZATION OF pso MUTANTS SENSITIVE TO PHOTO-ADDITION OF PSORALEN DERIVATIVES IN SACCHAROMYCES CEREVISIAE Genetics. ,vol. 95, pp. 273- 288 ,(1980) , 10.1093/GENETICS/95.2.273
Stephanie A. Nick McElhinny, Youri I. Pavlov, Thomas A. Kunkel, Evidence for Extrinsic Exonucleolytic Proofreading Cell Cycle. ,vol. 5, pp. 958- 962 ,(2006) , 10.4161/CC.5.9.2736
M. Moriya, Single-stranded shuttle phagemid for mutagenesis studies in mammalian cells: 8-oxoguanine in DNA induces targeted G.C-->T.A transversions in simian kidney cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 1122- 1126 ,(1993) , 10.1073/PNAS.90.3.1122
Sally Richards, Su-Ting Liu, Alokes Majumdar, Ji-Lan Liu, Rodney S Nairn, Michel Bernier, Veronica Maher, Michael M Seidman, Triplex targeted genomic crosslinks enter separable deletion and base substitution pathways Nucleic Acids Research. ,vol. 33, pp. 5382- 5393 ,(2005) , 10.1093/NAR/GKI851
Irina G. Minko, M. Todd Washington, Manorama Kanuri, Louise Prakash, Satya Prakash, R. Stephen Lloyd, Translesion synthesis past acrolein-derived DNA adduct, γ-hydroxypropanodeoxyguanosine, by yeast and human DNA polymerase η Journal of Biological Chemistry. ,vol. 278, pp. 784- 790 ,(2003) , 10.1074/JBC.M207774200
Patricia A. Dooley, Mingzhou Zhang, Gregory A. Korbel, Lubomir V. Nechev, Constance M. Harris, Michael P. Stone, Thomas M. Harris, NMR determination of the conformation of a trimethylene interstrand cross-link in an oligodeoxynucleotide duplex containing a 5'-d(GpC) motif. Journal of the American Chemical Society. ,vol. 125, pp. 62- 72 ,(2003) , 10.1021/JA0207798
Laura A. Fisher, Mika Bessho, Tadayoshi Bessho, Processing of a psoralen DNA interstrand cross-link by XPF-ERCC1 complex in vitro. Journal of Biological Chemistry. ,vol. 283, pp. 1275- 1281 ,(2008) , 10.1074/JBC.M708072200
Ivan D. Kozekov, Lubomir V. Nechev, M. Scott Moseley, Constance M. Harris, Carmelo J. Rizzo, Michael P. Stone, Thomas M. Harris, DNA interchain cross-links formed by acrolein and crotonaldehyde. Journal of the American Chemical Society. ,vol. 125, pp. 50- 61 ,(2003) , 10.1021/JA020778F