Mapping mammary gland architecture using multi-scale in situ analysis

摘要: We have built a novel computational microscopy platform that integrates image acquisition, storage, processing and analysis to study cell populations in situ. This allows high-content studies where multiple features are measured linked at scales. used this approach the cellular composition architecture of mouse mammary gland by quantitatively tracking distribution type, position, proliferative state, hormone receptor status epithelial cells incorporated bromodeoxyuridine while undergoing DNA synthesis during puberty retained label adult as function tissue structure. Immunofluorescence was identify label-retaining cells, well expressing proteinsprogesterone P63. Only 3.6% luminal were majority which did not express progesterone receptor. Multi-scale situ revealed distinct nuclear morphology, enriched 3.4-fold large ducts, distributed asymmetrically across tissue. postulated LRC ventral represent progenitor cells. Epithelial isolated from versus dorsal portion for putative stem markers CD24 CD49f fluorescence activated sorting. Thus, quantitative epithelium spatial scales identified previously unrecognized ventral-most, ducts contain reservoir undifferentiated,