Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25

摘要: Background Taste receptor cells are responsible for transducing chemical stimuli from the environment and relaying information to nervous system. Bitter, sweet umami utilize G-protein coupled receptors which activate phospholipase C (PLC) signaling pathway in Type II taste cells. However, it is not known how these communicate with Previous studies have shown that subset of expresses T2R bitter lack voltage-gated Ca2+ channels, normally required synaptic transmission at conventional synapses. Here we use two lines transgenic mice expressing green fluorescent protein (GFP) taste-specific promoters examine subsets cells: T1R3-GFP were used identify sweet- umami-sensitive cells, while TRPM5-GFP all PLC transduction. Voltage-gated currents assessed imaging whole cell recording, immunocytochemistry was detect expression SNAP-25, a presynaptic SNARE associated synapses