Acquisition of the H19 methylation imprint occurs differentially on the parental alleles during spermatogenesis.

摘要: The imprinted mouse H19 gene is hypomethylated on the expressed maternal allele and hypermethylated silent paternal allele. A 2-kb region of differential methylation located from -2 to -4 kb relative transcriptional start site has been proposed act as imprinting mark since hypermethylation in this inherited sperm retained throughout development. Here, we describe a temporal analysis patterns at locus during postnatal male germ cell methylated spermatogenesis, suggesting that acquired prior resumption mitosis mice. Likewise, more than half alleles are mitosis. However, remaining not until completion meiosis I, indicating de novo continuous process. Sequences proximal promoter, which spermatozoa somatic cells, differentially diploid, mitotic spermatogonia. becomes meiotic prophase. Thus, parental acquire germline.