Inhibition of CDK4 sensitizes multidrug resistant ovarian cancer cells to paclitaxel by increasing apoptosiss
作者： Yan Gao , Jacson Shen , Edwin Choy , Henry Mankin , Francis Hornicek
DOI: 10.1007/S13402-017-0316-X
关键词:
摘要: Overexpression of cyclin-dependent kinase (CDK) 4 has been observed in a variety cancers and found to contribute tumor cell growth proliferation. However, the effect inhibition CDK4 ovarian cancer is unknown. We investigated therapeutic inhibitor palbociclib combination with paclitaxel cells. Cell viabilities were determined by MTT assay after exposure different dosages and/or paclitaxel. Western blot, immunofluorescence, Calcein AM assays conducted determine mechanisms underlying cytotoxic effects siRNA was used validate outcome targeting that combinations significantly enhanced drug sensitivity both Rb-positive (SKOV3TR) Rb-negative (OVCAR8TR) cancer-derived When combined paclitaxel, induced apoptosis SKOV3TR OVCAR8TR also inhibited activity P-glycoprotein (Pgp), siRNA-mediated knockdown sensitized multidrug resistant (MDR) cells Inhibition can enhance MDR increasing apoptosis. may serve as promising target treatment cancer.
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