Prostaglandin endoperoxide synthase-1 and -2 couple to different transmembrane stimuli to generate prostaglandin D2 in mouse bone marrow-derived mast cells.

摘要: The view that the two isoforms of prostaglandin-endoperoxide synthase (cyclooxygenase), PGHS-1 and PGHS-2, mediate physiologic inflammatory processes, respectively, implies separate pathways arachidonic acid metabolism with different benefits to host. Functional segregation these steps in endogenous a single cell response stimuli is now demonstrated. When mouse bone marrow-derived mast cells developed interleukin-3 (IL-3)-containing medium were cultured c-kit ligand combination IL-10 IL-1 beta, transient expression PGHS-2 mRNA protein occurred dose- time-dependent fashion, accompanied by substantial release prostaglandin D2 (PGD2) into culture from 2 10 h. In contrast, induction did not an increase PGD2 generation stimulation IgE antigen. After longer period culture, 24 48 h, increased, as IgE/antigen-dependent PGD2. Dexamethasone, which inhibited but PGHS-1, PGHS-2-selective inhibitor suppressed cytokine-induced IgE-dependent generation. Thus, at time when both are present cells, they function independently coupling stimulus-initiated endogenously derived acid.