Advances in Proteomic Methods

摘要: It has been almost 20 years since the word proteome was coined in 1994. Proteomic techniques have experienced three generations: 2-DE MALDI-TOF, bottom-up, and top-down, although top-down not yet reached its full potential. Though numerous developed to assist achievement of accurate results large scale with high-throughput capability, field proteomics is still driven by development new technologies for sample processing, protein identification, quantification, structure, function. Advances preparation mainly focus on fractionation, enrichment, separation. Current trends aim obtain certain types peptides proteins or specific organelles. identification are aimed detect identify more accurately; while advances quantification focused improvement accuracy, precision, reproducibility. Structural functional begun, but require continued reach their In summary, every technique improve performance and/or reduce cost.