Repetitive titin epitopes with a 42 nm spacing coincide in relative position with known A band striations also identified by major myosin-associated proteins. An immunoelectron-microscopical study on myofibrils.

摘要: A direct titin-thick filament interaction in certain regions of the band is suggested by results using four new monoclonal antibodies specific for titin immunoelectron microscopy. Antibodies T30, T31 and T32 identify quasi-repeats molecule characterized a 42–43 nm repeat spacing. These stripes seem to coincide with striations established others on negatively stained cryosections band. T30 recognize epitopes matching five or two seven per half sacromere known harbor both myosin-associated C-protein an 86K (K = 10(3) Mr) protein. Antibody labels P zone, which correspond positions where decoration seen protein, but not C-protein. The single epitope defined antibody T33 located 55 prior center M This position seems M7 striation bands. proves that molecule, be anchored at Z line, also penetrates into complex architecture described here enable us begin correlate ultrastructural aspects interior part disposition sarcomere. They raise question whether there regular pattern between thick filaments.(ABSTRACT TRUNCATED AT 250 WORDS)