Cloning of the Arabidopsis ent-kaurene oxidase gene GA3
作者： C. A. Helliwell , C. C. Sheldon , M. R. Olive , A. R. Walker , J. A. D. Zeevaart
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摘要: The ga3 mutant of Arabidopsis is a gibberellin-responsive dwarf. We present data showing that the ga3-1 deficient in ent-kaurene oxidase activity, first cytochrome P450-mediated step gibberellin biosynthetic pathway. By using combination conventional map-based cloning and random sequencing we identified putative P450 gene mapping to same location as GA3. Relative progenitor line, two alleles contained single base changes generating in-frame stop codons predicted amino acid sequence P450. A genomic clone spanning locus complemented ga3-2 mutant. deduced GA3 protein defines an additional class enzymes. was expressed all tissues examined, RNA abundance being highest inflorescence tissue.
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