Activation of phospholipase D by glyceraldehyde in isolated islet cells follows protein kinase C activation.
作者： M Dunlop , S A Metz
DOI: 10.1210/ENDO.130.1.1727727
关键词:
摘要: Our recent studies have demonstrated the presence in neonatal islet cells and intact adult islets of a phosphatidylcholine-directed phospholipase D (PLD) which is activated after phorbol ester stimulation. The present study describes PLD activation carbohydrate insulin secretagogue. At highest concentration tested (20 mM) triose, glyceraldehyde, induced formation phosphatidic acid prelabeled with [14C]arachidonic or [3H]myristic (164 +/- 7 210 9% basal values, respectively). Experimental confirmation concentration-dependent specific was provided by transphosphatidylation product, phosphatidylethanol, stimulation glyceraldehyde added ethanol (1.5%). Additionally, there an early (within 5 min) rise [14C]arachidonate-labeled diacylglycerol (139 7% basal) accompanied increase intracellular mass (51 2 pmol/mg protein) a...
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