First evidence of Okadaic acid acyl-derivative and Dinophysistoxin-3 in mussel samples collected in Chiloe Island, Southern Chile.

摘要: This paper shows the detection of Diarrhetic Shellfish Poison (DSP) phycotoxins, using HPLC-FLD with pre-column derivatization procedure and HPLC-MS methods, in analysis shellfish extracts tested positive official DSP mouse bioassay. The samples were collected Chiloe Island, Southern Chile. amount Dinophysistoxin-3 (DTX-3) measured average above international safe limits for content analyzed. As internal control recovery, DTX-1 analytical standard was spiked into dichloromethane-clean order to calculate de extraction recovery DTX-1. 97%. From all toxins analyzed, hydrolyzed extract appeared mainly DTX-3 concentrations ranging from 99.40 +/- 1.22 257.73 12.46 ng/g digestive-glands. acyl-Okadaic Acid (acyl-OA) also detected some samples, 1.02 1.4 3.07. 1.6 ng toxin/g is first report acyl-OA ever found Chilean samples. data that contaminated a complex profile, which major toxin component, followed by as minor one. important findings showed this study are presence both acyl-derivates (DTX-3 Acyl-OA) product main metabolic biotransformation occurred inside shellfish, chelate OA, transforming them respectively. must be performed avoid self-inhibition their Protein Phosphatase 2A done since acyl-OA) do not inhibit 2A. profile permanent could explain diarrhea symptoms experience patients who have ingested cooked southern associated Vibrio parahaemolyticus or other enteropathogens had been suggested before. massive consumption an cultural habit now has become health issue