Comparative transcriptome profiling of healthy and diseased Chowghat Green Dwarf coconut palms from root (wilt) disease hot spots

摘要: Understanding the molecular basis of host-pathogen interactions is imperative for devising disease management strategies. The root (wilt) foremost debilitating threatening coconut production in India. To explore mechanisms involved compatible and incompatible interactions, global transcriptome profiling leaves healthy diseased Chowghat Green Dwarf (CGD) palms was conducted. RNA-Seq analysis generated more than 190 million 100 bp reads from both samples. Assembled yielded 59,282 transcripts with a median length 987 bp. BLASTX annotation resulted 39,665 getting annotated Uniprot date palm proteome database. Differential gene expression revealed 2718 to be up- or down- regulated comparison control at fold change 2 above p value <=0.05. differentially expressed could categorized into pathways which included cell wall biogenesis, primary secondary metabolism, plant-pathogen interaction, cellular transport, hormone biosynthesis signaling. Validation by quantitative real time PCR (RT-qPCR) set arbitrarily selected genes, up-regulated down-regulated, established comparable pattern as observed analysis. Overall, resources this study provide an in-depth new insights interaction pathogen.