Specific detection of monocytic lysozyme within normal and leukemic cells.
作者： C Leculier , N Couprie , A Francina , E Archimbaud , P Adeleine
DOI: 10.1182/BLOOD.V79.3.760.760
关键词:
摘要: A murine monoclonal antibody against human lysozyme (AHL MoAb) was produced and tested on normal leukemic monocytes using flow cytometry. The gave a positive reactivity permeabilized by saponin (82% to 98% of cells) negative neutrophils. This monocyte-specific had not been observed polyclonal antibody. Nevertheless, immunoblotting detected in both monocyte polymorphonuclear leukocyte (PMNL) lysates. AHL MoAb, the presence substrate (Micrococcus lysodeikticus cell walls), strongly inhibited enzymatic activity. Flow cytometric analysis cells isolated from patients suffering different subtypes acute myeloid leukemia (French-American-British [FAB] classification FAB M1–5) showed highly significant positivity M5 for compared with other subtypes. present results were consistent detection epitope MoAb located near catalytic site monocytes. same probably masked PMNL granules.
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