In silico analysis and expression profiling of miRNAs targeting genes of steviol glycosides biosynthetic pathway and their relationship with steviol glycosides content in different tissues of Stevia rebaudiana

摘要: miRNAs are emerging as potential regulators of the gene expression. Their proven promising role in regulating biosynthetic pathways related networks may hold key to understand genetic regulation these which assist selection and manipulation get high performing plant genotypes with better secondary metabolites yields increased biomass. associated genes steviol glycosides pathway, however, have not been identified so far. In this study targeting pathway were for first time whose precursors potentially generated from ESTs nucleotide sequences Stevia rebaudiana. Thereafter, stem-loop coupled real PCR based expressions different tissues rebaudiana investigated their relationship pattern was analysed expression levels target mRNAs well glycoside contents. All showed differential all three studied, viz. leaves, flowers stems. Out eleven validated, nine (miR319a, miR319b, miR319c, miR319d, miR319e, miR319f, miR319h, miRstv_7, miRstv_9) found be inversely related, while two, i.e. miR319g miRstv_11 on contrary, direct relation contents This provides a platform understanding can further employed manipulate biosynthesis enhance yield S. rebaudiana.