Studies of the regulation of the mouse carboxyl ester lipase gene in mammary gland.

摘要: The lactating mammary gland and pancreas of mouse constitute the main tissues for synthesis secretion a bile-salt-stimulated lipase called carboxyl ester (CEL). In this paper we have analysed endogenous CEL gene expression in gland. It is shown that expressed at day 14 pregnancy, which synchronous with whey acidic protein (WAP) gene. Even though WAP genes are induced same time during differentiation, their regulation different respect to dependence on lactogenic hormones. high induction due activation signal transducer activator transcription (STAT)5 by prolactin has not been observed gene, even it demonstrated both STAT5 isoforms interact one gamma-interferon sequence sites promoter Hence prolactin/STAT5 involved general significant 'milk genes'. Instead direct effect hormones, up-regulation correlated an increase number differentiated epithelial cells. Furthermore, studies using mammary-gland-derived cell line, HC11, show major positive element interacts member(s) CCAAT-binding factor/nuclear factor 1 family, binding palindromic site. Binding factor(s) important tissue-specific gland, because no was seen cells pancreatic origin.