Direct Reprogramming of Human Primordial Germ Cells into Induced Pluripotent Stem Cells: Efficient Generation of Genetically Engineered Germ Cells.

摘要: Primordial germ cells (PGCs) share many properties with embryonic stem (ESCs) and innately express several key pluripotency-controlling factors, including OCT4, NANOG, LIN28. Therefore, PGCs may provide a simple efficient model for studying somatic cell reprogramming to induced pluripotent (iPSCs), especially in determining the regulatory mechanisms that fundamentally define pluripotency. Here, we report novel of PGC generate iPSCs via transfection SOX2 OCT4 using integrative lentiviral. We also show feasibility nonintegrative approaches generating iPSC from only these two factors. human endogenous levels KLF4 C-MYC protein at similar (EGCs) but lower OCT4. Transfection both together was required induce state an efficiency 1.71%, further addition increased 2.33%. Immunohistochemical analyses SO-derived PGC-iPSCs revealed were more ESCs than EGCs regarding colony morphology molecular characterization. Although leukemia inhibitory factor (LIF) not generation like EGCs, presence LIF combined ectopic exposure yielded higher efficiencies. Additionally, exhibited differentiation into representative types all three layers vitro successfully formed teratomas vivo. Several lines generated karyotypically stable up 24 subcultures. Their derivation survival culture significantly supersedes demonstrating their utility as powerful factors regulating pluripotency future studies.