Inhibitory Potential of Prodomain of Plasmodium falciparum Protease Serine Repeat Antigen 5 for Asexual Blood Stages of Parasite
作者： Asrar Alam , Virander S Chauhan , None
DOI: 10.1371/JOURNAL.PONE.0030452
关键词:
摘要: Plasmodium falciparum serine repeat antigen 5 (SERA5) is a target for both drug and vaccine intervention against malaria. SERA5 secreted in the parasitophorous vacuole where it proteolytically processed before schizont rupture. Among products 50.8-kDa central domain of protease, which possesses chymotrypsin-like activity consists 28.9-kDa catalytic with 21.9-kDa N-terminal prodomain, remain attached together. Because has been implicated merozoite egress from host erythrocytes, effect prodomain heptapeptide derived its C-terminus spanning D560 to F566 (DNSDNMF) on parasite growth was studied. When E. coli-expressed incubated culture, significant delay transition ring stages observed up nanomolar concentrations. The peptide, DNSDNMF also showed similar effects but at nearly 1000-fold higher peptide found interact domain. These data demonstrate crucial role process. Given inhibitory potential parasite, we suggest that peptidomimetic inhibitors based sequences can be developed as future therapeutics
core.ac.uk
harvard.edu
paperity.org参考文章(32)
Elyzana D. Putrianti, Anja Schmidt-Christensen, Iris Arnold, Volker T. Heussler, Kai Matuschewski, Olivier Silvie, The Plasmodium serine-type SERA proteases display distinct expression patterns and non-essential in vivo roles during life cycle progression of the malaria parasite Cellular Microbiology. ,vol. 12, pp. 725- 739 ,(2009) , 10.1111/J.1462-5822.2009.01419.X
Susanne K. Miller, Robert T. Good, Damien R. Drew, Mauro Delorenzi, Paul R. Sanders, Anthony N. Hodder, Terence P. Speed, Alan F. Cowman, Tania F. de Koning-Ward, Brendan S. Crabb, A Subset of Plasmodium falciparum SERA Genes Are Expressed and Appear to Play an Important Role in the Erythrocytic Cycle Journal of Biological Chemistry. ,vol. 277, pp. 47524- 47532 ,(2002) , 10.1074/JBC.M206974200
Ahmed S.I. Aly, Kai Matuschewski, A malarial cysteine protease is necessary for Plasmodium sporozoite egress from oocysts. Journal of Experimental Medicine. ,vol. 202, pp. 225- 230 ,(2005) , 10.1084/JEM.20050545
Patrick Delplace, Bernard Fortier, Guy Tronchin, Jean-François Dubremetz, Alain Vernes, Localization, biosynthesis, processing and isolation of a major 126 kDa antigen of the parasitophorous vacuole of Plasmodium falciparum. Molecular and Biochemical Parasitology. ,vol. 23, pp. 193- 201 ,(1987) , 10.1016/0166-6851(87)90026-0
A. Podblelski, J. Hawlitzky, T. D. Pack, A. Flosdorff, M. D. P. Boyle, A group A streptococcal Enn protein potentially resulting from intergenomic recombination exhibits atypical immunoglobulin‐binding characteristics Molecular Microbiology. ,vol. 12, pp. 725- 736 ,(1994) , 10.1111/J.1365-2958.1994.TB01060.X
Joanna Trylska, Valentina Tozzini, Chia-en A. Chang, J. Andrew McCammon, HIV-1 Protease Substrate Binding and Product Release Pathways Explored with Coarse-Grained Molecular Dynamics Biophysical Journal. ,vol. 92, pp. 4179- 4187 ,(2007) , 10.1529/BIOPHYSJ.106.100560
Shuichi Kojima, Tetsuya Minagawa, Kin-ichiro Miura, Tertiary structure formation in the propeptide of subtilisin BPN′ by successive amino acid replacements and its close relation to function Journal of Molecular Biology. ,vol. 277, pp. 1007- 1013 ,(1998) , 10.1006/JMBI.1998.1671
Malcolm J. Gardner, Neil Hall, Eula Fung, Owen White, Matthew Berriman, Richard W. Hyman, Jane M. Carlton, Arnab Pain, Karen E. Nelson, Sharen Bowman, Ian T. Paulsen, Keith James, Jonathan A. Eisen, Kim Rutherford, Steven L. Salzberg, Alister Craig, Sue Kyes, Man-Suen Chan, Vishvanath Nene, Shamira J. Shallom, Bernard Suh, Jeremy Peterson, Sam Angiuoli, Mihaela Pertea, Jonathan Allen, Jeremy Selengut, Daniel Haft, Michael W. Mather, Akhil B. Vaidya, David M. A. Martin, Alan H. Fairlamb, Martin J. Fraunholz, David S. Roos, Stuart A. Ralph, Geoffrey I. McFadden, Leda M. Cummings, G. Mani Subramanian, Chris Mungall, J. Craig Venter, Daniel J. Carucci, Stephen L. Hoffman, Chris Newbold, Ronald W. Davis, Claire M. Fraser, Bart Barrell, Genome sequence of the human malaria parasite Plasmodium falciparum Nature. ,vol. 419, pp. 498- 511 ,(2002) , 10.1038/NATURE01097
Nobuko Arisue, Makoto Hirai, Meiji Arai, Hiroyuki Matsuoka, Toshihiro Horii, Phylogeny and evolution of the SERA multigene family in the genus Plasmodium. Journal of Molecular Evolution. ,vol. 65, pp. 82- 91 ,(2007) , 10.1007/S00239-006-0253-1
Jie Li, Hiroyuki Matsuoka, Toshihide Mitamura, Toshihiro Horii, Characterization of proteases involved in the processing of Plasmodium falciparum serine repeat antigen (SERA). Molecular and Biochemical Parasitology. ,vol. 120, pp. 177- 186 ,(2002) , 10.1016/S0166-6851(01)00452-2