Analysis of the Bovine Monocyte-Derived Macrophage Response to Mycobacterium avium Subspecies Paratuberculosis Infection Using RNA-seq.

摘要: Johne’s disease, caused by infection with Mycobacterium avium subsp. paratuberculosis, (MAP), is a chronic intestinal disease of ruminants serious economic consequences for cattle production in the United States and elsewhere. During infection, MAP bacilli are phagocytosed subvert host macrophage processes, resulting subclinical infections that can lead to immunopathology dissemination disease. Analysis transcriptome during therefore shed light on molecular mechanisms host-pathogen interplay associated Here we describe results an vitro study bovine monocyte-derived (MDM) response using RNA-seq. MDM were obtained from seven age- sex-matched Holstein-Friesian infected across six-hour time course non-infected controls. We observed 245 574 differentially expressed genes MAP-infected versus control samples (adjusted P value ≤ 0.05) at 2 6 hours post-infection, respectively. Functional analyses these genes, including biological pathway enrichment, highlighted potential functional roles have not been previously described bacilli. In addition, differential expression pro- anti-inflammatory cytokine such as those IL-10 signaling pathway, other immune-related encode proteins involved emphasize balance between protective immunity survival proliferation. Systematic comparisons RNA-seq gene Affymetrix® microarray data generated same experimental also demonstrated represents superior technology studying transcriptional responses intracellular infection.