Mouse singletons and twins developed from isolated diploid blastomeres supported with tetraploid blastomeres.
作者： Renata Czolowska , Waclaw Ozdzenski , Andrzej K. Tarkowski
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摘要: The aim of this study was to obtain mice, hopefully identical multiplets, from single diploid blastomeres isolated at the 4-cell stage, or pairs sister 8-cell stage. To end were aggregated with one two tetraploid carrier embryos produced by electrofusion 2-cell embryos. Diploid albino and homozygous for "a" allele glucose-phosphate isomerase (GPI-1a1a) pigmented GPI-1b1b. aggregates cultured in vitro up blastocyst Each quartet (occasionally triplet doublet) chimaeric blastocysts transplanted oviduct a separate pseudopregnant recipient. Altogether 62 17 recipients. Eight full-term foetuses (two singletons three twins) rescued Caesarian section on day 19, 20 21 pregnancy. Three young (one singleton successfully reared foster mothers proved be normal fertile females. All animals albino. In five individuals only 1-A form GPI (characteristic 2n blastomere) found. adult female traces 1-B 4n blastomeres) detected heart lungs while 4 other organs contained form. These observations strongly suggest that majority foetuses/animals according our experimental system are 'pure' diploids rather than 2n/4n chimaeras, described method can used future produce twins, triplets quadruplets mouse. Our confirms earlier work Kelly (1975, 1977) 'quarter' mouse still totipotent.
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