Early animal model evaluation of an implantable contrast agent to enhance magnetic resonance imaging of arterial bypass vein grafts

摘要: Background Non-invasive monitoring of autologous vein graft (VG) bypass grafts is largely limited to detecting late luminal narrowing. Although magnetic resonance imaging (MRI) delineates intima, media, and adventitia, which may detect early failure, the scan time required achieve sufficient resolution at present impractical. Purpose To study VG visualization enhancement in vivo delineate whether a covalently attached MRI contrast agent would enable quicker longitudinal wall. Material Methods Sixteen 12-week-old male C57BL/6J mice underwent carotid interposition grafting. The inferior vena cava nine donor was treated with gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA)-based agent, control VGs labeled vehicle. T1-weighted (T1W) performed serially postoperative weeks 1, 4, 12, 20. A portion animals sacrificed for histopathology following each point. Results signal-to-noise ratio (SNR) contrast-to-noise (CNR) were significantly higher in first three points (1.73 × higher SNR, P = 0.0006, 5.83 × higher CNR point, P = 0.0006). However, signal decreased consistently period, 1.29 × higher SNR 2.64 × higher CNR, by final There no apparent differences morphometric analyses Masson's trichrome-stained sections. Conclusion that binds wall provides significant increase T1W observed adverse effects mouse model. Further optimization enhance its durability required.