Model Studies for New o-Nitrobenzyl Photolabile Linkers: Substituent Effects on the Rates of Photochemical Cleavage.

摘要: Both a model phenacyl and o-nitrobenzyl photolabile linker from the literature along with four new linkers were prepared kinetics of their photolytic cleavage examined in solution. The by amidation carboxylic acid anchoring tether benzylamine, cleavable benzylic substituent was chosen to be either acetic or acetamide. Irradiation solvents (methanol, p-dioxane, aqueous buffer ± dithiothreitol) at 365 nm analysis via HPLC afforded kinetic rates suitable for comparative purposes. found cleave slowly under conditions no detectable being observed organic solvents. Known 4 showed modest Incorporation two alkoxy groups benzene ring generate veratryl-based 13a increased rate dramatically, introduction an additional methyl ...