Resonance Raman evidence of chloride binding to the heme iron in myeloperoxidase

摘要: The resonance Raman spectra of ferric derivatives myeloperoxidase at pH 8 show ligand-dependent differences. data are consistent with the resting enzyme and chloride fluoride all having 6-coordinated high-spin configurations. At 4 we find that is susceptible to photodegradation from our low power incident laser beam. Chloride binding inhibits this denaturation. Our support direct under physiological conditions.