Unsuitability of Using Ribosomal RNA as Loading Control for Northern Blot Analyses Related to the Imbalance between Messenger and Ribosomal RNA Content in Rat Mammary Tumors

作者： Montserrat Solanas , Raquel Moral , Eduard Escrich

DOI: 10.1006/ABIO.2000.4889

关键词:

摘要:

参考文章(7)

Hua Zhong, Jonathan W. Simons, Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia. Biochemical and Biophysical Research Communications. ,vol. 259, pp. 523- 526 ,(1999) , 10.1006/BBRC.1999.0815

P. Bhatia, W.R. Taylor, A.H. Greenberg, J.A. Wright, Comparison of Glyceraldehyde-3-phosphate Dehydrogenase and 28S-Ribosomal RNA Gene Expression as RNA Loading Controls for Northern Blot Analysis of Cell Lines of Varying Malignant Potential Analytical Biochemistry. ,vol. 216, pp. 223- 226 ,(1994) , 10.1006/ABIO.1994.1028

Pierre R. Provost, Yves Tremblay, Standardization of Northern Blot Signals by Determination of Relative Poly(A)+ RNA Amounts Loaded per Lane Analytical Biochemistry. ,vol. 277, pp. 154- 156 ,(2000) , 10.1006/ABIO.1999.4381

Scott C. Supowit, Jeffrey M. Rosen, Gene expression in normal and neoplastic mammary tissue. Biochemistry. ,vol. 19, pp. 3452- 3460 ,(1980) , 10.1021/BI00556A008

Piotr Chomczynski, Nicoletta Sacchi, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Analytical Biochemistry. ,vol. 162, pp. 156- 159 ,(1987) , 10.1016/0003-2697(87)90021-2

Andrej-Nikolai Spiess, Richard Ivell, Normalization of RNA hybridization signals by means of SYBR green II-stained 28S or 18S ribosomal RNA and a phosphor imager. BioTechniques. ,vol. 26, pp. 46- 50 ,(1999) , 10.2144/99261BM06

Redmer Da, Johnson Ml, Reynolds Lp, Quantification of lane-to-lane loading of poly(A) RNA using a biotinylated oligo(dT) probe and chemiluminescent detection BioTechniques. ,vol. 19, pp. 712- 715 ,(1995)