Effects of Ginsenoside Rg1 Regulating Wnt/β-Catenin Signaling on Neural Stem Cells to Delay Brain Senescence.
作者： Yue Xiang , Shun-he Wang , Lu Wang , Zi-ling Wang , Hui Yao
DOI: 10.1155/2019/5010184
关键词:
摘要: This is a study on the relationship between protective effect of ginsenoside Rg1 senescent neural stem cells and Wnt-β/catenin signaling pathway. Background. Recent studies have shown that overactivation Wnt/β-catenin pathway closely related to cell senescence. Whether delays senescence NSCs regulation this Methods. The whole brain Nestin-GFP transgenic newborn rat was extracted, were extracted cultured P3 generation. following indicators detected: (1) NSC culture identification, (2) LiCl proliferation survival rate NSCs, (3) (4) growth in each group observed by an optical microscope, (5) cycle detected flow cytometry, (6) proliferative ability BrdU, (7) fluorescence intensity Nestin Sox2 (8) positive staining analyzed SA-β-Gal staining, (9) localization β-catenin laser confocal microscopy, (10) changes pathway-related proteins Western blotting. Results. activates promotes mouse Ginsenoside inhibits activation. Conclusions. can activate antagonize caused activation delay aging.
hindawi.com参考文章(35)
Yunjoon Jung, Andrew S. Brack, Cellular Mechanisms of Somatic Stem Cell Aging Current Topics in Developmental Biology. ,vol. 107, pp. 405- 438 ,(2014) , 10.1016/B978-0-12-416022-4.00014-7
W. Zhang, G.M. Wang, P.J. Wang, Q. Zhang, S.H. Sha, Effects of neural stem cells on synaptic proteins and memory in a mouse model of Alzheimer's disease. Journal of Neuroscience Research. ,vol. 92, pp. 185- 194 ,(2014) , 10.1002/JNR.23299
Pam Ellis, B. Matthew Fagan, Scott T. Magness, Scott Hutton, Olena Taranova, Shigemi Hayashi, Andrew McMahon, Mahendra Rao, Larysa Pevny, SOX2, a Persistent Marker for Multipotential Neural Stem Cells Derived from Embryonic Stem Cells, the Embryo or the Adult Developmental Neuroscience. ,vol. 26, pp. 148- 165 ,(2004) , 10.1159/000082134
Gerd Kempermann, Sebastian Jessberger, Barbara Steiner, Golo Kronenberg, Milestones of neuronal development in the adult hippocampus Trends in Neurosciences. ,vol. 27, pp. 447- 452 ,(2004) , 10.1016/J.TINS.2004.05.013
Da-yong Zhang, Hai-jie Wang, Yu-zhen Tan, Wnt/β-Catenin Signaling Induces the Aging of Mesenchymal Stem Cells through the DNA Damage Response and the p53/p21 Pathway PLoS ONE. ,vol. 6, pp. e21397- ,(2011) , 10.1371/JOURNAL.PONE.0021397
Hans Clevers, Roel Nusse, Wnt/β-catenin signaling and disease. Cell. ,vol. 149, pp. 1192- 1205 ,(2012) , 10.1016/J.CELL.2012.05.012
Jiahong Zhu, Xinyi Mu, Jin Zeng, Chunyan Xu, Jun Liu, Mengsi Zhang, Chengpeng Li, Jie Chen, Tinyu Li, Yaping Wang, Ginsenoside Rg1 Prevents Cognitive Impairment and Hippocampus Senescence in a Rat Model of D-Galactose-Induced Aging PLoS ONE. ,vol. 9, pp. e101291- ,(2014) , 10.1371/JOURNAL.PONE.0101291
Christin Glorioso, Etienne Sibille, Between destiny and disease: genetics and molecular pathways of human central nervous system aging. Progress in Neurobiology. ,vol. 93, pp. 165- 181 ,(2011) , 10.1016/J.PNEUROBIO.2010.11.006
Wong RN Leung KW, Yung KK, Mak NK, Chan YS, Fan TP, Neuroprotective effects of ginsenoside-Rg1 in primary nigral neurons against rotenone toxicity Neuropharmacology. ,vol. 52, pp. 827- 835 ,(2007) , 10.1016/J.NEUROPHARM.2006.10.001
Maria A Blasco, None, Telomere length, stem cells and aging Nature Chemical Biology. ,vol. 3, pp. 640- 649 ,(2007) , 10.1038/NCHEMBIO.2007.38