Acyl type of bacterial cell wall: Its simple identification by colorimetric method.

作者: KINYA UCHIDA , KÔ AIDA

DOI: 10.2323/JGAM.23.249

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摘要: A simple and practical method was studied to identify the acyl type of bacterial cell wall. This is based on determination glycolic acid derived from hydrolysis a small amount cells. The devised system with micro-columns useful for quantitative separation complex materials in hydrolysate, determined by colorimetric Calkins. Experiments showed that about 50 60nmol glycolyl residue present 1mg dry cells strains such as Corynebacterium equi AJ 1402 (ATCC 6939), Brevibacterium imperiale 1446 (IAM 1654), Brev. testaceum 1464 1537), but scarcely found Coryn. diphtheriae 1414 11913), Nocardia madurae 9136 (NRRL B-2127), so on. No group other than acetyl residues or only detected purified wall various bacteria tested.From these results it concluded are classified into relative their cell-wall type, which can be easily decided estimation whole

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