Physical characteristics of ribosomal protein S4 from Escherichia coli.
作者： J. Dodd , W.E. Hill
DOI: 10.1016/S0021-9258(18)61529-7
关键词:
摘要: Abstract A hydrodynamic study of protein S4 from Escherichia coli 30 S ribosomal subunits indicates that this is moderately asymmetric. sedimentation coefficient 1.69 and a diffusion 7.58 X 10(-7) cm2/s suggest has an axial ratio about 5:1 using prolate ellipsoidal model. This structure should give radius gyration 29-30 small-angle neutron or x-ray scattering studies. utilized quasi-elastic light as analytical tool to obtain well method monitor sample quality. Using in manner allows assessment problems associated with purity which may be responsible for the many disparate results reported proteins especially S4.
sci-hub.st 参考文章(32)
E. Kaltschmidt, H.G. Wittmann, Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Analytical Biochemistry. ,vol. 36, pp. 401- 412 ,(1970) , 10.1016/0003-2697(70)90376-3
Charles Tanford, Physical Chemistry of Macromolecules ,(1961)
L.G. Tumanova, A.T. Gudkov, V.N. Bushuev, M.S. Okon, Study of the tertiary structure of protein L11 from escherichia coli ribosomes in solution by proton magnetic resonance FEBS Letters. ,vol. 127, pp. 245- 249 ,(1981) , 10.1016/0014-5793(81)80215-3
David A. Yphantis, Equilibrium Ultracentrifugation of Dilute Solutions Biochemistry. ,vol. 3, pp. 297- 317 ,(1964) , 10.1021/BI00891A003
Jennifer A. Littlechild, Ailsa L. Malcolm, A new method for the purification of 30S ribosomal proteins from Escherichia coli using nondenaturing conditions. Biochemistry. ,vol. 17, pp. 3363- 3369 ,(1978) , 10.1021/BI00609A029
Y.Gloria Chu, Charles R. Cantor, Segmental flexibility in Escherichia coli ribosomal protein S1 as studied by fluorescence polarization Nucleic Acids Research. ,vol. 6, pp. 2363- 2379 ,(1979) , 10.1093/NAR/6.6.2363
Esin Gulari, Erdogan Gulari, Y. Tsunashima, B. Chu, Photon correlation spectroscopy of particle distributions Journal of Chemical Physics. ,vol. 70, pp. 3965- 3972 ,(1979) , 10.1063/1.437950
V. Ramakrishnan, M. Capel, M. Kjeldgaard, D.M. Engelman, P.B. Moore, Positions of proteins S14, S18 and S20 in the 30 S ribosomal subunit of Escherichia coli. Journal of Molecular Biology. ,vol. 174, pp. 265- 284 ,(1984) , 10.1016/0022-2836(84)90338-3
H. Hasko PARADIES, Alfred FRANZ, Geometry of the Protein S4 from Escherichia coli Ribosomes FEBS Journal. ,vol. 67, pp. 23- 29 ,(1976) , 10.1111/J.1432-1033.1976.TB10627.X
I.N. Serdyuk, M.A. Sarkisyan, Z.V. Gogia, Compact structure of ribosomal protein S4 in solution as revealed by small-angle x-ray scattering. FEBS Letters. ,vol. 129, pp. 55- 58 ,(1981) , 10.1016/0014-5793(81)80754-5