Cordycepin Downregulates Cdk-2 to Interfere with Cell Cycle and Increases Apoptosis by Generating ROS in Cervical Cancer Cells: in vitro and in silico Study.

摘要: Background Cordycepin is a small molecule from medicinal mushroom Cordyceps, which has been reported for anticancer properties. Objective In this study, we have focused on the investigation of cordycepin effect cervical cancer cells with further clarification possible molecular mechanism. Method We used cell viability and counting assay cytotoxic cordycepin, flow cytometric apoptosis cycle, quantitative PCR (qPCR) Western blotting determination target gene expression. Molecular docking dynamics simulation were in silico analysis affinity to protein(s). Results Treatment controlled SiHa HeLa growth, increased rate their apoptosis, interfered specifically elongated S-phase. qPCR results indicated that there was downregulation cycle proteins CDK-2, CYCLIN-A2 CYCLIN-E1 mRNA level by treatment but no significant change observed pro-apoptotic or antiapoptotic proteins. The intracellular reactive oxygen species (ROS) treated significantly, implying might be induced ROS. blot confirmed decrease Cdk-2 mild Cyclin-E1 Cyclin-A2 responsible regulating cycle. high binding against Cdk-2. docked pose cordycepin-Cdk-2 complex remained within pocket 10 ns. Conclusion Our study suggests effective cells, via proteins, especially downregulating Cdk-2, inducing generating ROS are among mechanisms activities cordycepin.