Crosstalk between HSF1 and HSF2 during the heat shock response in mouse testes

摘要: Heat Shock Factor 1 (HSF1) is the primary transcription factor responsible for response to cellular stress, while HSF2 becomes activated during development and differentiation, including spermatogenesis. Although both factors are indispensable proper spermatogenesis, activation of HSF1 by heat shock initiates apoptosis spermatogenic cells leading infertility males. To characterize mechanisms assisting such induced we studied how cooperate response. For this purpose used chromatin immunoprecipitation proximity ligation approaches. We looked co-occupation binding sites in untreated (32 °C) or shocked (at 38 °C 43 spermatocytes, which most sensitive hyperthermia. At physiological temperature after mild hyperthermia at °C, sharing HSFs was observed mainly promoters Hsp genes other stress-related genes. Strong resulted an increased releasing HSF2, hence promoter regions not detected any more. The close (and/or existence HSF1/HSF2 complexes) frequent temperature. Temperature elevation a decreased number complexes they were barely strong °C. have concluded that cells. However, causes remodeling interactions between disrupted. This potentially affects regulation stress contributes sensitivity these