A Mouse Model for Binge-Level Methamphetamine Use.

摘要: Binge/crash cycles of methamphetamine (MA) use are frequently reported by individuals suffering from MA disorders. An binge is self-reported as multiple daily doses that commonly accumulate to 800 mg/day (~10 mg/kg/day for a 170 pound human). A genetic animal model with similar vulnerability binge-level intake missing. We used selectively bred high drinking (MAHDR) and low (MALDR) mouse lines determine whether several procedural variations would result in intake. Data were also collected two progenitor populations the lines, DBA/2J (D2) strain F2 cross D2 C57BL/6J strains. The impact 3 factors was examined: (1) concentration two-bottle choice procedure selective breeding; (2) ratio bottles containing vs. water, (3) length withdrawal (or abstinence) period between sessions. When progressively increased every 4 days 20 mg/l amounts 140 mg/l, maximum MALDR mice 1.1 mg/kg, whereas MAHDR consumed much 14.6 mg/kg. these concentrations tested bottle procedure, highest water (3:1) associated escalated up 29.1 mg/kg 12.0 mice; did not show ratio-dependent escalation Finally, offered at increasing 80 under an intermittent 6-h period, which lengthened 30 hours (D2 mice) or 78 (MAHDR). initially 14-16 MA, but reduced their 3-fold after introduction 30-h abstinence periods, retained level regardless period. provide appropriate study MA-induced neurobiological changes pharmaceutical treatments.