R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein, RGS9-1.
作者： G. Hu , T. G. Wensel
关键词:
摘要: Abstract The regulator of G protein signaling (RGS)-9-1⋅Gβ5 complex forms the GTPase accelerating for Gαt in vertebrate photoreceptors. Although is soluble when expressed vitro, extraction endogenous from membranes requires detergents. The detergent extracts contain a RGS9-1, Gβ5, Gαt, and 25-kDa phosphoprotein, R9AP (RGS9-1-Anchor Protein). encoded by one intronless gene both human mouse. Full or partial cDNA genomic clones were obtained mice, cattle, human, zebrafish, Xenopus laevis. mRNA was detected only retina, binds to N-terminal domain anchors it disk membrane via C-terminal transmembrane helix.
harvard.edu
core.ac.uk
sci-hub.se 参考文章(46)
T.A. Neubert, R.S. Johnson, J.B. Hurley, K.A. Walsh, The rod transducin alpha subunit amino terminus is heterogeneously fatty acylated. Journal of Biological Chemistry. ,vol. 267, pp. 18274- 18277 ,(1992) , 10.1016/S0021-9258(19)36955-8
Scott P. Heximer, Han Lim, Jennifer L. Bernard, Kendall J. Blumer, Mechanisms governing subcellular localization and function of human RGS2. Journal of Biological Chemistry. ,vol. 276, pp. 14195- 14203 ,(2001) , 10.1074/JBC.M009942200
Mathew E. Sowa, Wei He, Kevin C. Slep, Michele A. Kercher, Olivier Lichtarge, Theodore G. Wensel, Prediction and confirmation of a site critical for effector regulation of RGS domain activity. Nature Structural & Molecular Biology. ,vol. 8, pp. 234- 237 ,(2001) , 10.1038/84974
T G Wensel, J K Angleson, Enhancement of rod outer segment GTPase accelerating protein activity by the inhibitory subunit of cGMP phosphodiesterase. Journal of Biological Chemistry. ,vol. 269, pp. 16290- 16296 ,(1994) , 10.1016/S0021-9258(17)34006-1
Z Yang, T.G. Wensel, N-myristoylation of the rod outer segment G protein, transducin, in cultured retinas. Journal of Biological Chemistry. ,vol. 267, pp. 23197- 23201 ,(1992) , 10.1016/S0021-9258(18)50076-4
Z. Rahman, S. J. Gold, M. N. Potenza, C. W. Cowan, Y. G. Ni, W. He, T. G. Wensel, E. J. Nestler, Cloning and Characterization of RGS9-2: A Striatal-Enriched Alternatively Spliced Product of the RGS9 Gene The Journal of Neuroscience. ,vol. 19, pp. 2016- 2026 ,(1999) , 10.1523/JNEUROSCI.19-06-02016.1999
Abraham Kovoor, Henry A. Lester, Gi Irks GIRKs Neuron. ,vol. 33, pp. 6- 8 ,(2002) , 10.1016/S0896-6273(01)00572-4
C. W. Cowan, R. N. Fariss, I. Sokal, K. Palczewski, T. G. Wensel, High expression levels in cones of RGS9, the predominant GTPase accelerating protein of rods Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 5351- 5356 ,(1998) , 10.1073/PNAS.95.9.5351
Ching-Kang Chen, Marie E. Burns, Wei He, Theodore G. Wensel, Denis A. Baylor, Melvin I. Simon, Slowed recovery of rod photoresponse in mice lacking the GTPase accelerating protein RGS9-1 Nature. ,vol. 403, pp. 557- 560 ,(2000) , 10.1038/35000601
Hermann Schägger, Gebhard von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry. ,vol. 166, pp. 368- 379 ,(1987) , 10.1016/0003-2697(87)90587-2