Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy

摘要: Abstract Light scattering by brain tissue and phototoxicity are major obstacles to the use of high-resolution optical imaging photo-activation (‘uncaging’) bioactive compounds from inactive (‘caged’) precursors in intact semi-intact nervous systems. Optical methods based on 2-photon excitation promise reduce these (Denk, 1994; Denk et al., 1990, 1994). Here we show a range modes laser scanning microscopy (TPLSM) as applicable problems neuroscience. Fluorescence images were taken neurons labeled with ion-sensitive voltage-sensitive dyes invertebrate ganglia, mammalian slices, brain. Scanning photochemical whole-cell current detection 1994) how distribution neurotransmitter receptors surface specific cells can be mapped. All strong sectioning usable obtained at depths greater than 100 μm below preparation.