Further Evidence for the Genetic Heterogeneity of Clostridium botulinum as Determined by 23S rDNA Oligonucleotide Probing

摘要: Summary A stretch of the 23S rDNA from Clostridium botulinum strains that produce different types neurotoxins was sequenced and three oligonucleotide probes designed. They were labelled non-radioactively with DIG-ddUTP tested for specificity by dot blot hybridization against rRNA 83 clostridia other Gram-positive organisms. The discriminated between groups C. defined their toxin thus supporting previous results on DNA-DNA DNA-rRNA similarity studies phenotypic characterization: (i) proteolytic toxins A, B F, as well closely related sporogenes putrificum a strain barati produces type F; (ii) non-proteolytic E; D, (iii) argentinense (formerly ) G, subterminale . These extend spectrum published recently B, E F ( Campbell et al., 1993).