Fish Erythrocytes as Biomarkers for the Toxicity of Sublethal Doses of an Azo Dye, Basic Violet-1 (CI: 42535)

摘要: The aim of the present study was to investigate poikilocytosis in Labeo rohita (an important food fish) as an early indicator stress due azo dye, Basic Violet-1 (CI: 42535). This dye observed be very toxic test fish (96 h LC50 as0.45 mg/L dye). Fish were given short-term h) and subchronic (150 days) exposures recorded under light scanning electron microscopy (SEM). Light helped identification micronuclei along with irregularities, notches, blebs, lobes, crenation, clumps, chains, spherocytes, vacuolation, necrosis erythrocytes. However, SEM indicated shrinkage, oozing cytoplasm, several new abnormal shapes including marginal foldings, discocytes, keratocytes, dacrocytes, degmacytes, acanthocytes, echinocytes, protuberances, stomatocytes, drepanocytes, holes membrane, stippling/spicules, crescent-shaped cells, triangular pentagonal cells. Earlier studies speculated changes membrane responsible for clumping chaining erythrocytes, whereas clearly indicates that out cytoplasm is also formation chains clumps. shows erythrocytes exhibit pathological symptoms before appearance other external such behavior or mortality fish. There a dose- duration-dependent increase; therefore, poikilocytosis, especially can act biomarker caused by dyes.