Silencing early viral replication in macrophages and dendritic cells effectively suppresses flavivirus encephalitis.
作者： Chunting Ye , Sojan Abraham , Haoquan Wu , Premlata Shankar , N. Manjunath
DOI: 10.1371/JOURNAL.PONE.0017889
关键词:
摘要: West Nile (WN) and St. Louis encephalitis (SLE) viruses can cause fatal neurological infection currently there is neither a specific treatment nor an approved vaccine for these infections. In our earlier studies, we have reported that siRNAs be developed as broad-spectrum antivirals the of caused by related small peptide called RVG-9R deliver siRNA to neuronal cells well macrophages. To increase repertoire antiflaviviral siRNAs, screened 25 targeting conserved regions in viral genome. Five were found inhibit both WNV SLE replication vitro reflecting antiviral activity one was also validated vivo. addition, show delivers macrophages dendritic cells, resulting effective suppression virus replication. Mice challenged intraperitoneally (i.p.) with (WNV) treated i.v. siRNA/peptide complex. The peritoneal isolated on day 3 post transferred new hosts. receiving from anti-viral mice failed develop any disease while control irrelevant all died encephalitis. These studies suggest early RVG-9R-mediated delivery key preventing development disease.
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